![]() Acquisition of data, analysis and interpretation, beyond routine practices.Conception, design of project, critical input, or original ideas.Please list our flow core staff members as a co-author if we're involved in one of the following areas (based on the authorship guidelines from the Association of Biomolecular Resources Facilities published in May 2010): Please email us the Pubmed ID for the publication with the above acknowledgement. Investigators who do not comply with this NIH requirement may lose access to the core services. These studies used the resources of the Diabetes and Endocrinology Research Center Flow Core Facility funded in part through Center Grant 5P30DK063608. Please use the below wording in every publication which results from the use of the flow core instruments. The hands-on session (2 hours) includes the instrument startup/shutdown, optical filter selection, software usage, PMT voltage adjustment, data storage, and troubleshooting.Īs the recipient of four NIH awards, we are required to acknowledge the NIH funding source in all publications (manuscripts, abstracts, presentations, etc.).Fluorofinder panel design web-based tool for CCTI/HICCC cytometers and instruction.Consultation session will be conducted on panel design and protocol suggestion.Web-based theory session (2 hours) covers the principle of flow cytometry, components of flow cytometry (optics, fluidic, electronic), optical measurement, data analysis and sorting.The data in network drive are erased every few days, again, WITHOUT prior notice. Non-CCTI members, use your CUIMC provided OneDrive directory here. It is the SOLE responsibility of all flow core users to backup their data RIGHT AFTER the data acquisition. For CCTI members, save all data to your CCTI P: share or OneDrive. fcs files and templates frequently WITHOUT prior notice. The flow core DOES NOT backup any data, and to facilitate the smooth operation of the Diva and other flow software, deletes all. If you don't find yourself among researchers listed in the above web pages, but you belong to one of the groups, please contact the core director. Herbert Irving Comprehensive Cancer Center.Department of Dermatology research faculty. ![]() Department of Medicine research faculty.We define your affiliation based on the following: Users from other departments can only reserve 48 hours in advance. Reservationsįor those who affiliate with the CCTI, Cancer Center, Department of Medicine, DRC, or SDRC, you can reserve our instrument 3 weeks in advance. Please contact the core for generating the online instrument reservation account. Cell sorter is operated by dedicated core facility staff only. ![]() If the user already has experience in operation of the instrument, a 5-10 minutes briefing sessions about the general maintenance of the analyzers would be given. AnalyzersĮmail Caisheng Lu (CCTI Site) to discuss which instrument best fits the experiment and training will be arranged accordingly. Please contact the facility staff for any issues regarding training, assistance and access with flow including protocol design, instrument operation, data analysis, and troubleshooting. Among the many documented applications, characterization, and quantification of cells for surface protein, cytokine, intracellular transcription factor, and signaling molecules are the most common experiments performed in the flow cytometry core facility. The Flow Cytometry Core of the Columbia Center for Translational Immunology (CCTI) and Herbert Irving Comprehensive Cancer Center (HICCC) provides training and access to state-of-the-art flow cytometry for biomedical investigators at the Columbia University Irving Medical Center.
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